Journal: Journal of Oral Microbiology

Article Title: Macrophage activation and invasion by P. gingivalis is modulated by PPAD and accessory fimbriae subunits

doi: 10.1080/20002297.2026.2638646

Figure Lengend Snippet: LPS contamination in fimbriae preparations is negligible and does not influence the pro-inflammatory activation of MDMs. A) MDMs were treated for 24 h with purified fimbriae (1 μg/ml) from ATCC 33277 WT (fimbriae WT) and ∆PPAD mutant (fimbriae ∆PPAD) strains, as well as LPS-free fimbriae from both strains; n = 3. B) hMDMs were preincubated for 30 min with control (IgG1) or TLR4-blocking antibody (anti-TLR4), then treated for 24 h with purified fimbriae (1 μg/ml) from ATCC 33277 WT (fimbriae WT) and the ∆ ppad mutant (fimbriae ∆PPAD) strains; n = 3. Levels of IL-6 and IL-8 secretion were subsequently measured. Data are presented as mean ± SEM. ‘ns’ indicates not statistically significant. Statistically significant differences are shown in A) between LPS-free fimbriae and fimbriae without the LPS removal step; in B) between IgG1 and anti-TLR4 pretreated samples.

Article Snippet: Cells were treated for 30 min with a TLR4 neutralising antibody or mouse IgG1 isotype control (both from Invivogen) at 5 μg/mL before treatment with fimbriae.

Techniques: Activation Assay, Purification, Mutagenesis, Control, Blocking Assay

Journal: Science Advances

Article Title: A beneficial environment promotes immune resilience through epigenetic regulation

doi: 10.1126/sciadv.ady7317

Figure Lengend Snippet: ( A ) Airway resistance measured after increasing concentrations of nebulized methacholine to anesthetized mice. ( B ) Representative BAL differential counts and PAS staining images and ( C ) quantification of BAL eosinophilia and ( D ) volume of mucus within airway epithelium. ( E ) Reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis from whole-lung homogenates detecting the expression of Il13 gene. ( F ) Serum titers of anti-OVA–specific IgE. ( G ) Annotated immune cells were subsetted from whole-lung scRNA-seq and 2D plotted using uniform manifold approximation and projection for dimension reduction (UMAP). ( H ) OVA and OVA + FD immune cell UMAP representation overlaid. ( I ) DEG number comparing OVA versus OVA + FD groups. ( J ) Representative flow cytometry plots highlighting MDM and DC identification and ( K and L ) quantification of frequency of these cell types within parent gates. Results are plotted as means ± SEM, and difference between means were compared using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test ( n = 6 to 9).

Article Snippet: Mouse CCL8 (BioLegend, no. 446904), human CCL8 (BioLegend, no. 442204), OVA-specific IgE (Chondrex, no. 3004), and OVA-specific IgG1 (Chondrex, no. 3013) were used according to the supplier’s protocols.

Techniques: Staining, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Flow Cytometry